Lion's Mane Mushrooms from Sawdust Spawn | Key Growing Information

Lion's Mane Mushroom Spawn
CULTURE: Use sawdust spawn to grow Lion's Mane mushrooms on logs, stumps, pillars (also known as "totems"), or in containers. Yields will be greatest when growing Lion's Mane on pillars, and fastest when growing them in containers. Mushrooms require ample moisture. Choose a location for your production that is easy to access and monitor and is at least partially shaded year-round. Easy access to a water source may also be beneficial. Please note that mushroom production in different climates may require altered practices, some options for which are detailed below.

Remove spawn bags from shipping box promptly. Untuck bags to expose the filter patch and increase air flow. If mushroom fruiting bodies have already developed on the filter patch, carefully remove them without causing damage to the filter and bag. Mushroom growth begins from mycelium (a vegetative structure made up of whitish fibers). In sawdust spawn bags, this can appear as a fuzzy growth resembling mold, but not to worry; it is healthy growth. If spawn needs to be stored prior to inoculation, refrigerate for up to 6 months, but fresh spawn will have the best chance of success. Do not open the bag prior to the day of inoculation as it will significantly heighten the risk of contamination.

Lion's Mane requires more patience than other species: logs and stumps may begin fruiting in 1–2 years; pillars may produce within a year; and containers can begin fruiting in just a few months. Time to fruiting is always subject to variable environmental conditions, but it can also be affected by substrate type or tree species and the ratio of spawn to substrate. Fruiting happens only once mycelium has been fully established (colonized) in substrate. In mushroom production on logs, smaller logs take less time to fully colonize than large logs, and stumps may take even longer.

Lion's Mane is primarily a wood-loving species, but it can also be grown in containers. See our chart: Substrate Types for Growing Mushrooms for more information. Lion's Mane is slower than some others to colonize, so it is important to source fresh material. Fresher substrate carries a lower risk of contamination (pests, and other fungi) than substrate allowed to age.

Some tree species are better suited to certain mushroom strains. Lion's Mane is best suited to growing on beech, hornbeam, and hard maple, but has a relatively wide range of suitability. See our chart: Suitability of Tree Species for Growing Mushrooms on Logs for more information. Healthy trees with intact bark can be harvested at any time of year except in spring between bud swell and full leaf-out when the tree's energy is supporting developing leaves. The ideal time is in the month prior to bud swell.

For production on logs, cut trees of at least 3" diameter to desired length. Logs should be inoculated within a week or two of cutting unless they can be stored in a way that keeps them below freezing and hydrated, e.g., covered in snow during northern winters. Using a 12 mm bit, drill holes of 1" depth every 4–6" in rows 2–3" apart, staggered in a diamond pattern down the length of the log. Break up sawdust spawn. Use a log inoculation tool by jabbing the end into the spawn a few times, then lining it up with the holes in the log. Depress the thumb piece to insert spawn into each hole, ensuring the top is flush with or slightly below the surface of the log. Using daubers or a paint brush, seal plugged holes with melted wax. This protects the log both from drying out and potential contamination. If growing in a dry climate, consider also sealing the cut ends of the logs with wax.

For pillar production, cut trees of a larger diameter into at least 2 lengths of 6–18" and at least one 2" long to serve as a cap. Logs should be inoculated within a week or two of cutting unless they can be stored in a way that keeps them below freezing and hydrated, e.g., covered in snow during northern winters. Lay a piece of cardboard on the ground and add a 1" layer of spawn in a circle roughly the same diameter as the logs. Place a 6–18" piece on top of the spawn. On the surface of the log piece, add another 1" layer of sawdust spawn and stand another log piece on that. Add a final 1" layer of sawdust spawn and cap with a 2" piece. The number of log pieces and therefore height of a pillar is limited only by the structural integrity of the arrangement.

Sawdust spawn can be used to inoculate freshly cut stumps of a suitable tree species. This method has the potential to yield mushrooms for a significantly longer time than logs. Follow the instructions for growing on logs to produce mushrooms on stumps with the following additions: make sure to first girdle the stump by removing the outer bark in a ring around the base to prevent suckers from growing. In the inoculation step, also drill holes in the cut surface of the stump.

Mushrooms can be grown in containers indoors or outdoors. Lion's Mane will only grow from the side of a container. Use a container that already has side openings like a milk or bulb crate, or drill holes if the container does not already have them. Holes of ½" will allow mushrooms to fruit but minimizes moisture loss during colonization. To reduce risk of contamination, sanitize containers before inoculation. Substrate may also be pasteurized to kill off potential contaminants using either cold water with hydrated lime or hot water. To inoculate, start with moist substrate. Ideally, this would be around 50% capacity, or when the substrate weighs twice what it did when dry. Next, break up and mix spawn, adding between ½ bag and 1 full bag of spawn to 5 gallons of substrate. A higher ratio of spawn leads to faster and more reliable colonization and is recommended for beginner growers. Add mixture to the desired container, mixing thoroughly or making alternating layers of substrate and spawn.

Maintaining moisture levels is paramount during the time between inoculation and fruiting, when mycelium grows, but no fruiting bodies are visible. Allow this process to happen in an area out of direct sunlight. If producing on pillars or in containers, consider covering with a tarp, a layer of straw mulch, or similar material to keep moisture in. Remove cover when mushrooms are ready to begin fruiting, indicated by pins (baby mushrooms) beginning to form on the surface. If producing on logs, keep the logs close to the ground but not in direct contact with it, and stack densely to help mitigate moisture loss due to excessive airflow.

When logs are fully colonized, sometimes indicated by the presence of mycelium on the cut ends, they should be rearranged in a way that increases aeration and harvester access. Fruiting will naturally occur the first time. After the initial fruiting, if more regular harvests are desired, logs may be "force fruited" by soaking them in cold water for no more than 24 hours. This can be attempted with Lion's Mane but works best with Shiitake. Allow about 8 weeks between flushes if using this method.

Maturing mushrooms may grow very quickly and should be checked at least once a day when nearing maturity. To harvest, twist or cut fruiting body off at its base. Lion's Mane fruiting body will initially appear as a dense white form. Tooth-like structures begin to develop when they are near maturity. Harvest when teeth are 1/4– 1/2" long and before they start to yellow. Overmature mushrooms will have a shorter shelf life and are more easily damaged in handling. Fading or discoloration, as well as firmness, are indicators that the mushrooms may not be ideal for consumption, as their flavor and texture can degrade with age.

When growing mushrooms using any method, the number of flushes produced will depend on substrate density and type and environmental conditions. When growing on logs, the larger the diameter, the longer the production window. Due to their diameter, stumps and pillars have the potential to produce for much longer than logs. In northern climates, a rule of thumb is that the log's diameter in inches equals the number of years it is likely to produce. This timeline will be shorter in mild areas with a more continuous growing season. Containers and their substrate vary too greatly to estimate yield.

Mold can grow on sawdust spawn damaged in transit or on harvested mushrooms that have been stored for a long time. Do not consume moldy mushrooms. To combat slug damage, diatomaceous earth can be spread on beds or around the base of logs. Mammals can be a minor pest but often will not consume all your yield. Mammals can be deterred by using row cover such as Agribon-15.

Ensure mushrooms are cooked thoroughly before consumption. If eating a species for the first time, start with a small amount to test for allergies. Logs or substrate for mushrooms grown outside have a higher risk of being colonized by other fungal species. Make sure to confidently identify mushroom species prior to consumption, as they may be a fruiting body of a local species.